NY Study: Risk Factors for Mortality in Patients with COVID-19 in New York City

Source: Springer Link

Abstract

Background

New York City emerged as an epicenter of the coronavirus disease 2019 (COVID-19) pandemic.

Objective

To describe the clinical characteristics and risk factors associated with mortality in a large patient population in the USA.

Design

Retrospective cohort study.

Participants

6493 patients who had laboratory-confirmed COVID-19 with clinical outcomes between March 13 and April 17, 2020, who were seen in one of the 8 hospitals and/or over 400 ambulatory practices in the New York City metropolitan area

Main Measures

Clinical characteristics and risk factors associated with in-hospital mortality.

Key Results

A total of 858 of 6493 (13.2%) patients in our total cohort died: 52/2785 (1.9%) ambulatory patients and 806/3708 (21.7%) hospitalized patients. Cox proportional hazard regression modeling showed an increased risk of in-hospital mortality associated with age older than 50 years (hazard ratio [HR] 2.34, CI 1.47–3.71), systolic blood pressure less than 90 mmHg (HR 1.38, CI 1.06–1.80), a respiratory rate greater than 24 per min (HR 1.43, CI 1.13–1.83), peripheral oxygen saturation less than 92% (HR 2.12, CI 1.56–2.88), estimated glomerular filtration rate less than 60 mL/min/1.73m² (HR 1.80, CI 1.60–2.02), IL-6 greater than 100 pg/mL (HR 1.50, CI 1.12–2.03), D-dimer greater than 2 mcg/mL (HR 1.19, CI 1.02–1.39), and troponin greater than 0.03 ng/mL (HR 1.40, CI 1.23–1.62). Decreased risk of in-hospital mortality was associated with female sex (HR 0.84, CI 0.77–0.90), African American race (HR 0.78 CI 0.65–0.95), and hydroxychloroquine use (HR 0.53, CI 0.41–0.67).

Conclusions

Among patients with COVID-19, older age, male sex, hypotension, tachypnea, hypoxia, impaired renal function, elevated D-dimer, and elevated troponin were associated with increased in-hospital mortality and hydroxychloroquine use was associated with decreased in-hospital mortality.

INTRODUCTION

The coronavirus disease 2019 (COVID-19) is a pandemic that has impacted medical systems, societies, and economies worldwide. The first case of COVID-19, caused by severe acute respiratory syndrome 2 virus (SARS-CoV-2)¹, was reported in China in December 2019². The virus has spread globally at a rapid pace, resulting in more than 2 million confirmed cases as of April 17, 2020³. In recent weeks, New York City has emerged as an epicenter of the pandemic, with over 120,000 confirmed cases and over 13,000 deaths due to confirmed or probable COVID-19 death as of April 17, 2020⁴. Studies of the clinical characteristics and epidemiologic characteristics of COVID-19 have been conducted in countries experiencing outbreaks earlier than the USA^{5,6,7,8,9,10,11}. Large-scale observational data of the clinical characteristics and outcomes of COVID-19 in the population of the USA are scarce. In this study, we describe the clinical characteristics of COVID-19 in ambulatory and inpatient settings and identify risk factors associated with mortality in hospitalized patients.

METHODS

Study Design and Participants

A multicenter retrospective cohort study of patients with COVID-19 patients was conducted using the medical records of the Mount Sinai Health System, a large urban health system of 8 hospitals and more than four hundred ambulatory practices in the New York City metropolitan area. Patients with a positive SARS-CoV-2 test result and an encounter with a healthcare provider for COVID-19 between March 12 and April 17, 2020, were included in this study. A confirmed case of COVID-19 was defined as a positive result on reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay of nasopharyngeal swab specimens. The study population was dichotomized into ambulatory and hospitalized groups. The former included patients whose encounter was an office visit, emergency department (ED) visit, or telehealth/telemedicine. Inpatients and ambulatory patients who were subsequently admitted to the hospital were included in the hospitalized group.

Both groups were further subdivided into survivors and non-survivors. Ambulatory non-survivors were patients who had expired prior to presentation to the ED, who had expired in the ED prior to admission to the hospital units, or who had an office or telemedicine encounter and were later found out to be deceased. Ambulatory survivors included all other ambulatory patients. Hospitalized non-survivors were patients who had expired as of April 17, 2020. Hospitalized survivors were patients who had been discharged home or to other facilities as of April 17, 2020.

Icahn School of Medicine at Mount Sinai has waived informed consent and Institutional Review Board approval because the study used a de-identified database.

Definitions

The following covariates were extracted from the database: patients’ age, sex, ethnicity, race, smoking status, vital signs including temperature, peripheral oxygen saturation (SpO₂), heart rate, respiratory rate (RR), blood pressure (BP), body mass index (BMI), and laboratory results including white blood cell count (WBC), D-dimer, interleukin-6 (IL-6), hemoglobin, estimated glomerular filtration rate (eGFR), alanine aminotransferase (ALT), aspartate aminotransferase (AST), C-reactive protein (CRP), procalcitonin, ferritin, lactate dehydrogenase (LDH), fibrinogen (FBG), interleukin-6 (IL-6), comorbidities, and treatments.

Statistical Analysis

Continuous variables were reported as median with interquartile range. Categorical variables were expressed as proportions. Temporary changes of vital signs and laboratory values in survivors and non-survivors for the first 14 days after admission were assessed.

To illustrate the risk associated with changes in the continuous variables, including vital signs and laboratory values, multivariate generalized additive models were used to calculate the odds ratio (OR) for mortality, with each median value set as a reference (i.e., OR = 1). The hazard ratio (HR) of each variable for mortality risk was assessed using univariate Cox proportional hazard regression model.

To account for missing data values for laboratory results, we introduced multiple imputation, which is a procedure used to replace missing values with other plausible values by creating multiple filling-in patterns to avert bias caused by missing data. Using the dataset with imputed values, univariate and multivariate Cox model were fit to calculate HR.

The multivariate Cox model was adjusted for the following variables assessed in the univariate Cox model: patients’ age, sex, race, cigarette use history, past medical history of asthma, hypertension, diabetes, or cancer, systolic BP, RR, SpO₂, BMI, initial laboratory values (lymphocyte proportion, D-dimer, IL-6), and hydroxychloroquine use.

For this Cox regression analysis, we excluded variables from the univariable analysis if their between-group differences were not significant, if the number of events was too small to calculate hazard ratios, or if they had collinearity with other significant values. Each hospital was considered by the clustering term in the Cox proportional hazard model analysis where the clustering effect associated with hospitals was accounted for by the robust sandwich estimator.

Preliminary confirmation of predictability of the Cox proportional hazard model demonstrated the area under the curve (AUC) to be 0.808 (95% CI, 0.790–0.825, Supplementary Figure 1). To investigate the effect of hydroxychloroquine while addressing the imbalance among treatment groups, we introduced inverse probability weighting (IPTW) based on propensity scoring to control for observed differences in baseline characteristics between treatment group and control group.

IPTW was calculated based on the same variables as used in the Cox regression models, except for hydroxychloroquine use. We then fitted an IPTW-adjusted Cox with doubly robust methods. Survival curves with stratification for hydroxychloroquine were constructed using the Kaplan-Meier method. All statistical analyses were performed using version 3.6.2 of the R programming language (R Project for Statistical Computing; R Foundation).

RESULTS

Demographic and Clinical Characteristics

Between March 13 and April 17, there were 6493 confirmed COVID-19 cases, including 2785 (42.9 %) ambulatory patients and 3708 (57.1%) hospitalized patients. The demographics, clinical characteristics, and laboratory findings are shown in Table 1. The median age of the group was 59 (interquartile range [IQR] 43 to 72) with 66.6% of the patients older than 50 years of age. 45.5% of the patients were female. Based on patients’ self-reported race, 26.9% were white, 24.1% were African American, 4.4% were Asian, and 44.7% were other. Based on self-reported ethnicity, 57.5% were Non-Hispanic, 25.4% were Hispanic, and the rest were unknown or not reported.

Table 1 Clinical Characteristics of the Patients with COVID-19 Full size table

Ambulatory and Hospitalized Comparison

The median age was 47 years old in the ambulatory group (IQR 34 to 60) and 66 years old in the hospitalized group (IQR 55 to 78). 858 patients died (13.2%): 52 patients in the ambulatory group (1.9%) and 806 patients in the hospitalized group (21.7%). Among ambulatory patients, 69% were emergency room encounters without hospital admission, 18.2% were office-based encounters, and 1.4% were telemedicine encounters.

Compared with that of ambulatory patients, a higher proportion of hospitalized patients were older, were male, or had a history of cigarette use. Hospitalized patients were more likely to have coexisting medical conditions including asthma, chronic obstructive pulmonary disease (COPD), hypertension, obesity, diabetes mellitus (DM), chronic kidney disease (CKD), and cancer. Hospitalized patients were more likely to have abnormal vital signs and abnormal laboratory values including higher WBC count, lymphocyte, and neutrophil counts, higher levels of AST, CRP, procalcitonin, ferritin, IL-6, LDH, D-dimer, and troponin, and lower levels of eGFR and hemoglobin. Clinical characteristics of hospitalized patients stratified by age group, gender, race, and hydroxychloroquine use are shown in Supplementary Tables 2, 3, 4, and 5, respectively.

Survivors and Non-Survivors

Clinical characteristics of the 2014 survivors and 806 non-survivors in the hospitalized group are shown in Table 2 (Supplementary Table 1 for the ambulatory group).

The median number of days to discharge for survivors was 5 days (IQR, 3 to 9 days). The median number of days to death for non-survivors was also 5 days (IQR, 3 to 9 days). Compared with survivors, non-survivors were older and the higher proportion were male.

Non-survivors were more likely to have a history of cigarette use and coexisting medical conditions including COPD, hypertension, DM, and CKD.

Table 2 Clinical Characteristics of the Hospitalized Patients with COVID-19Full size table

Temporal changes of vital signs and laboratory values in survivors and non-survivors during hospitalization are shown in Figure 1. Throughout hospitalization, non-survivors had higher heart rate and respiratory rate and lower oxygen saturation compared with survivors. Initial laboratory findings of non-survivors demonstrated higher WBC count and higher levels of D-dimer, IL-6, AST, CRP, procalcitonin, ferritin, LDH, fibrinogen, and troponin. Throughout hospitalization, non-survivors had higher WBC count, neutrophil proportion, LDH, and ferritin levels, and lower eGFR and lymphocyte proportion. Non-survivors also had higher levels of CRP, D-dimer, and IL-6 in the first week of hospitalization. Non-survivors showed a marked increase in LDH, CRP, D-dimer, AST, ALT, and procalcitonin on day 1 after admission. Both groups had a trend of decreasing hemoglobin levels and increasing platelet counts during hospitalization; however, a more pronounced decrease in hemoglobin levels was seen in non-survivors, while an increase in platelet counts was greater for survivors. The generalized additive models demonstrated correlations between laboratory values and increased odds of in-hospital mortality which are similar to the difference observed between hospitalized survivors and non-survivors (Supplementary Figure 3).

Treatment

The majority of hospitalized patients received hydroxychloroquine (74.6% of survivors and 71.3% of non-survivors) and azithromycin (67.4% of survivors and 71.3% of non-survivors). Fewer hospitalized patients received other medications such as remdesivir, anakinra, tocilizumab, or sarilumab (Table 2). The majority of ambulatory patients did not receive hydroxychloroquine or azithromycin.

Kaplan-Meier estimate showed lower mortality in hospitalized patients who received hydroxychloroquine (log rank P value < 0.001) (Supplementary Figure 4).

Risk Factors Associated with Mortality in Hospitalized Patients

The results of multivariate Cox proportional hazard regression models are shown in Table 3 (univariate models are shown in Supplementary Table 6). Of 3708 hospitalized patients, 888 patients remained hospitalized as of April 7 and were not included in the analysis. In the multivariate analysis, factors associated with a higher risk of in-hospital mortality included age over 50, systolic blood pressure less than 90 mmHg, a respiratory rate greater than 24 per min, SpO₂less than 92%, eGFR less than 60 mL/min/1.73m², IL-6 greater than 100 pg/mL (6.5 times upper limit of normal [ULN]), D-dimer greater than 2 mcg/mL (4 times ULN), and troponin greater than 0.03 ng/mL. Factors associated with a lower risk of in-hospital mortality included female sex, African American race, and hydroxychloroquine use. The adjustment with IPTW did not lead to a significant change in the HR of hydroxychloroquine (without IPTW: HR 0.53, CI 0.41–0.67; with IPTW: HR 0.53, CI 0.41–0.68).

Table 3 Risk Factors Associated with In-Hospital Death Full size table

DISCUSSION

We report a large retrospective cohort study of both ambulatory and hospitalized patients with COVID-19 from across the New York City metropolitan area. The clinical characteristics described here represent the first large retrospective cohort study from the US population in a city at the epicenter of the pandemic.

Early reports showed that COVID-19 had a mortality rate among all confirmed cases of 2%¹² which is significantly lower compared with that of 34% with MERS¹³ and 10% with SARS¹⁴. The mortality rate in hospitalized patients reported previously ranged from 4 to 28%^{2, 7,8,9, 11}. The mortality rate of 25.9% among hospitalized patients in our study may be explained by more severe disease in our total cohort, by a different reporting method, or by geographic variation.

We identified several risk factors associated with mortality in hospitalized patients with COVID-19 that have been previously reported including older age and male sex. We report additional risk factors associated with in-hospital mortality including low SBP, tachypnea, low SpO₂, low eGFR, and higher levels of IL-6, D-dimer, and troponin levels.

The severity of coronavirus infection in humans has been previously described to increase during viral clearance suggesting pathogenicity arising from host immune response¹⁵. Our study confirmed again that older patients with COVID-19 hospitalization are at significantly higher risk of mortality. We did not observe any independent association between in-hospital mortality and some of the common coexisting medical conditions including hypertension, diabetes, or cancer. However, using calculated GFR as a surrogate for CKD, we observed that decreased renal function was a risk factor for in-hospital mortality, a finding that is consistent with previous studies¹⁶.

IL-6 and other pro-inflammatory cytokines production are felt to be due to immune dysregulation rather than normal response to SARS-CoV infection^17, 18. Our findings are consistent with this theory, and we observed elevated IL-6 as an independent prognostic risk factor, with higher levels in non-survivors. In hospitalized patients, we saw fluctuating IL-6 levels, with a significant increase seen on day 1 of admission and an increasing level trend that was more pronounced in non-survivors.

Thrombocytosis was associated with disease activity in SARS and was thought to be secondary to the direct effect of the virus or effect of inflammatory cytokines¹⁹. We observed a greater thrombocytosis during hospitalization in survivors than in non-survivors. A previous study of IL-6 in primates revealed that there is a dose-dependent response of thrombocytosis induced by IL-6²⁰. The discrepancy between high IL-6 levels and lack of thrombocytosis in non-survivors could be explained by endothelial damage and subsequent platelet consumption from viral infection, impaired platelet release from megakaryocytes in the lung, or direct impairment of hematopoiesis²¹. This may suggest that the absence of reactive thrombocytosis may portend a poor response to SARS-CoV-2 infection.

Elevated D-dimer in COVID-19 patients has been described previously^22, 23. We report in this study its independent association with an increased risk of in-hospital mortality. Abnormal D-dimer alone is non-specific; however, the higher elevation in non-survivors suggests that coagulopathy, particularly disseminated intravascular coagulation (DIC), may contribute to mortality in COVID-19.

One of the functional receptors for pathogenic human coronavirus such as SARS-CoV is angiotensin-converting enzyme 2 (ACE2)²⁴, and these receptors are expressed in heart tissues²⁵. This suggests that SARS-CoV-2 virus could directly affect the heart. Similar to the previous finding that showed an association of cardiac injury and a higher risk of in-hospital mortality²⁶, we observed elevated troponin levels in hospitalized patients as a risk factor for increased mortality.

Hydroxychloroquine is an analog of chloroquine, a widely used anti-malarial with immunomodulatory effects²⁷. In vitro studies have shown that hydroxychloroquine has activity against SARS-CoV-2 ²⁸. The clinical data of hydroxychloroquine in patients with COVID-19 come from small studies that have shown mixed results. Chen et al. randomized 30 hospitalized patients with COVID-19 to receive hydroxychloroquine 400 mg daily for 5 days or placebo and found that 86.7% of the hydroxychloroquine group and 93.3% of the control group had negative throat swabs²⁹. Chen et al. randomized 62 patients to hydroxychloroquine or placebo and reported shortened time to clinical recovery, fever resolution, and cough improvement in the hydroxychloroquine group³⁰. Mahevas et al. reviewed 181 hospitalized patients with COVID-19 data who received hydroxychloroquine 600 mg daily and reported no difference in outcomes, including in ICU admission and/or death at 7 days follow-up³¹. Another randomized trial of 150 hospitalized patients by Tang et al. did not show symptomatic improvement at 28 days or clearance of SARS-CoV-2 with hydroxychloroquine use³². We attempted to adjust for all known confounders between the groups who did and did not receive hydroxychloroquine using multivariate regression analyses and the IPTW method, which revealed that hydroxychloroquine use was associated with decreased risk of in-hospital mortality. Due to the inherent limitations of our retrospective study design, there was no conclusive determination on the efficacy of hydroxychloroquine in patients with COVID-19. More robust studies such as randomized clinical trials are needed.

Our study has several limitations. First, we have no long-term follow up data for ambulatory and discharged patients; hence, the clinical outcome observed may not be reflective of the true eventual outcome, particularly in the ambulatory group. Second, we have patients who remained hospitalized at the time of our analyses and did not have our outcomes, such as discharge or mortality, and were excluded for our comparison of survivors and non-survivors. Third, due to limitations and local testing policy during the study duration, there are an unknown number of patients who were not diagnosed with COVID-19 because of a lack of severe symptoms and/or hospitalization. Fourth, we are not able to adjust for unknown confounders that may affect the true treatment effect. These limitations prevent any definitive conclusions on the efficacy of any treatment.

CONCLUSIONS

In this retrospective study of over 6000 ambulatory and hospitalized patients with COVID-19 in the New York City metropolitan area, age, male sex, tachypnea, low systolic blood pressure, low peripheral oxygen saturation, impaired renal function, elevated IL-6, elevated D-dimer, and elevated troponin were found to be risk factors for mortality. Hydroxychloroquine use was associated with decreased mortality.

References

1. Zhou P, Yang XL, Wang XG, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579(7798):270-273.CAS Article Google Scholar 
2. Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497-506.
3. World Health Organization. Coronavirus Disease (COVID-19) Pandemic. https://www.who.int. Accessed 04/18, 2020.
4. New York City Department of Health and Mental Hygiene. COVID-19 Data. https://www1.nyc.gov/site/doh/covid/covid-19-data.page. Accessed 04/18, 2020.
5. Grasselli G, Zangrillo A, Zanella A, et al. Baseline Characteristics and Outcomes of 1591 Patients Infected With SARS-CoV-2 Admitted to ICUs of the Lombardy Region, Italy. JAMA. 2020.Google Scholar 
6. Guan WJ, Ni ZY, Hu Y, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med. 2020.
7. Chen N, Zhou M, Dong X, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020;395(10223):507-513.CAS Article Google Scholar 
8. Chen T, Wu D, Chen H, et al. Clinical characteristics of 113 deceased patients with coronavirus disease 2019: retrospective study. BMJ. 2020;368:m1091.Article Google Scholar 
9. Wang D, Hu B, Hu C, et al. Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China. JAMA. 2020.
10. Xu XW, Wu XX, Jiang XG, et al. Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series. BMJ. 2020;368:m606.Article Google Scholar 
11. Zhou F, Yu T, Du R, et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet. 2020;395(10229):1054-1062.CAS Article Google Scholar 
12. Wu Z, McGoogan JM. Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China: Summary of a Report of 72314 Cases From the Chinese Center for Disease Control and Prevention. JAMA. 2020.
13. World Health Organization. Middle East respiratory syndrome coronavirus (MERS-CoV). https://www.who.int/emergencies/mers-cov/en. Accessed 04/18, 2020.
14. World Health Organization. Cumulative Number of Reported Probable Cases of Severe Acute Respiratory Syndrome (SARS). https://www.who.int/csr/sars/country/en/. Accessed 04/18, 2020.
15. Perlman S, Netland J. Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Microbiol. 2009;7(6):439-450.CAS Article Google Scholar 
16. Henry BM, Lippi G. Chronic kidney disease is associated with severe coronavirus disease 2019 (COVID-19) infection. Int Urol Nephrol. 2020.
17. Thiel V, Weber F. Interferon and cytokine responses to SARS-coronavirus infection. Cytokine Growth Factor Rev. 2008;19(2):121-132.CAS Article Google Scholar 
18. Perlman S, Dandekar AA. Immunopathogenesis of coronavirus infections: implications for SARS. Nat Rev Immunol. 2005;5(12):917-927.CAS Article Google Scholar 
19. Wong RS, Wu A, To KF, et al. Haematological manifestations in patients with severe acute respiratory syndrome: retrospective analysis. BMJ. 2003;326(7403):1358-1362.Article Google Scholar 
20. Asano S, Okano A, Ozawa K, et al. In vivo effects of recombinant human interleukin-6 in primates: stimulated production of platelets. 1990.Google Scholar 
21. Yang M, Ng MH, Li CK. Thrombocytopenia in patients with severe acute respiratory syndrome (review). Hematology. 2005;10(2):101-105.Article Google Scholar 
22. Han H, Yang L, Liu R, et al. Prominent changes in blood coagulation of patients with SARS-CoV-2 infection. Clin Chem Lab Med. 2020.
23. Lippi G, Favaloro EJ. D-dimer is Associated with Severity of Coronavirus Disease 2019: A Pooled Analysis. Thromb Haemost. 2020.
24. Li W, Moore MJ, Vasilieva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426(6965):450-454.CAS Article Google Scholar 
25. Gallagher PE, Ferrario CM, Tallant EA. Regulation of ACE2 in cardiac myocytes and fibroblasts. Am J Physiol Heart Circ Physiol. 2008;295(6):H2373-2379.CAS Article Google Scholar 
26. Shi S, Qin M, Shen B, et al. Association of Cardiac Injury With Mortality in Hospitalized Patients With COVID-19 in Wuhan, China. JAMA Cardiol. 2020.Google Scholar 
27. Keyaerts E, Vijgen L, Maes P, Neyts J, Van Ranst M. In vitro inhibition of severe acute respiratory syndrome coronavirus by chloroquine. Biochem Biophys Res Commun. 2004;323(1):264-268.CAS Article Google Scholar 
28. Yao X, Ye F, Zhang M, et al. In Vitro Antiviral Activity and Projection of Optimized Dosing Design of Hydroxychloroquine for the Treatment of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Clin Infect Dis. 2020.
29. Chen J, Liu L, Liu P, Xu Q, Xia L, Ling Y, Huang D, Song S, Zhang D, Qian Z, Li T, Shen Y, Lu H. A pilot study of hydroxychloroquine in treatment of patients with common coronavirus disease-19 (COVID-19). J Zhejiang Univ (Med Sci). 2020;49(1):0-0.
30. Chen Z, Hu J, Zhang Z, et al. Efficacy of hydroxychloroquine in patients with COVID-19: results of a randomized clinical trial. medRxiv. 2020:2020.2003.2022.20040758.
31. Mahevas M, Tran V-T, Roumier M, et al. No evidence of clinical efficacy of hydroxychloroquine in patients hospitalized for COVID-19 infection with oxygen requirement: results of a study using routinely collected data to emulate a target trial. medRxiv. 2020:2020.2004.2010.20060699.
32. Tang W, Cao Z, Han M, et al. Hydroxychloroquine in patients with COVID-19: an open-label, randomized, controlled trial. medRxiv. 2020:2020.2004.2010.20060558.

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Acknowledgments

We thank the Mount Sinai Data Warehouse team for the COVID-19 database, and Norihiro Inoue MD, PhD, Hiroki Ueyama MD, Satoshi Miyashita MD, Misato Nagumo MD, and Mizuho Asada PhD for giving us critical comments and input.

Authors’ Roles

TM and ES had the idea for and designed the study and had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. TM and ES drafted the paper. TM, ES, HM, and TY did the analysis, and all authors critically revised the manuscript for important intellectual content and gave final approval for the version to be published. TM and ES collected the data. All authors agree to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Author information

Affiliations

1. Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USATakahisa Mikami M.D., Hirotaka Miyashita M.D., Takayuki Yamada M.D., Matthew Harrington M.D., Daniel Steinberg M.D., Andrew Dunn M.D. & Evan Siau M.D.
2. Department of Medicine, Mount Sinai Beth Israel, 281 First Ave, Box #218, New York, 10003, NY, USATakahisa Mikami M.D., Hirotaka Miyashita M.D., Takayuki Yamada M.D., Matthew Harrington M.D., Daniel Steinberg M.D. & Evan Siau M.D.
3. Department of Medicine, Mount Sinai Hospital, New York, NY, USAAndrew Dunn M.D.

Corresponding author

Correspondence to Evan Siau M.D..

Ethics declarations

Informed consent was waived because of the de-identified and retrospective nature of the data. We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this work is consistent with those guidelines.

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The authors declare that they do not have a conflict of interest.

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